貨號： 10777019 規(guī)格 Product Size: 5,000 units Shipping Condition: Dry Ice 描述 RNaseOUT? 重組核糖核酸酶抑制劑 (RNaseOUT? Recombinant Ribonuclease Inhibitor) 是胰型核糖核酸酶例如 RNase A 的強效非競爭性抑制劑。如同其它 RNase 抑制劑，RNaseOUT? 重組核糖核酸酶抑制劑是酸性蛋白質(zhì)，分子量約為 52 kDa。 RNaseOUT? 抑制 RNase A、RNase B 和 RNase C。應(yīng)用：cDNA 合成、RT-PCR (2)，以及體外轉(zhuǎn)錄和翻譯。來源： 通過親和色譜從表達克隆的豬基因的大腸桿菌 (E. coli ) 中純化。性能和質(zhì)量測試： SDS-PAGE 純度、內(nèi)切脫氧核糖核酸酶測定、蛋白質(zhì)濃度、比活；經(jīng)過 RT-PCR 性能評估。單位定義： 使用胞苷 2´, 3´ 環(huán)單磷酸 (cCMP) 作為底物，一個單位可對 5 ng 的 RNase A 產(chǎn)生 50% 的抑制效果 (1)。單位反應(yīng)條件： 反應(yīng)物共 1 ml，包含 100 mM Tris-醋酸 (pH 6.5)、1 mM EDTA、0.2 mM cCMP、2 µg RNase A，反應(yīng)時間 0 ...
發(fā)布時間:
2015
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05
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26
瀏覽次數(shù)：211
貨號： 13778150 規(guī)格 Cell Type: Established Cell Lines, Hard-to-Transfect Cells, Primary Cells, Stem Cells Sample Type (Specific): Synthetic siRNA Transfection Technique: Lipid-Based Transfection Serum Compatible: Yes Product Size: 1.5 mL Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice High Throughput Compatibility: High Throughput-Compatible Green Features: Sustainable packaging 內(nèi)容及儲存 每套包含一管試劑。置于+4°C保存。Lipofectamine® RNAiMAX轉(zhuǎn)染試劑正確儲存時可穩(wěn)定保存1年。 描述 Lipofectamine? RNAiMAX轉(zhuǎn)染試劑可在多種細胞類型中獲得最高的轉(zhuǎn)染效率，適用于siRNA介導(dǎo)的基因抑制實...
發(fā)布時間:
2015
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05
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26
瀏覽次數(shù)：392
貨號： 18064014 規(guī)格 Optimal Reaction Temperature: 42° C Final Product Size(s): 12.3 kb or less Reverse Transcriptase: SuperScript® II Ribonuclease H Activity: Reduced Form: Frozen Format: Tube(s) Template: ssDNA, ssRNA, RNA-DNA Hybrid Sensitivity: Medium Product Size: 10,000 units Concentration: 200 U?µl Enzyme Function: RNA dependent DNA Polymerase Shipping Condition: Wet Ice Number of Reactions: 50 Reactions 內(nèi)容及儲存 SuperScript® II Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl...
發(fā)布時間:
2015
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05
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26
瀏覽次數(shù)：117
貨號： 11765054 規(guī)格 Form: Liquid Volume: 500 ml Glutamine: L-Glutamine HEPES Buffer: No HEPES Product Size: 500 mL Phenol Red Indicator: Phenol Red Sodium Pyruvate Additive: Sodium Pyruvate Green Features: Sustainable packaging Shipping Condition: Room Temperature 描述 Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial...
發(fā)布時間:
2015
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05
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26
瀏覽次數(shù)：127
貨號： 12183018A 規(guī)格 Sample Type (General): Liquid Samples (e.g. Serum), Bacteria, Yeast, Cells, Plant Samples, Tissue, Blood High Throughput Compatibility: Not High Throughput-Compatible (Manual) Downstream Application: Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nuclease Protection Assays, Next-Generation Sequencing, Nucleic Acid Labeling, Microarray Analysis, Real-Time Quantitative PCR (qPCR), Northern Blotting Format: Spin Column System: PureLink? Product Size: 50 preps Final Product: Total RN...
發(fā)布時間:
2015
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05
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26
瀏覽次數(shù)：481