貨號：?18064014
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規(guī)格
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| Optimal Reaction Temperature: | 42° C |
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| Final Product Size(s): | 12.3 kb or less |
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| Reverse Transcriptase: | SuperScript? II |
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| Ribonuclease H Activity: | Reduced |
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| Form: | Frozen |
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| Format: | Tube(s) |
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| Template: | ssDNA,?ssRNA,?RNA-DNA Hybrid |
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| Sensitivity: | Medium |
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| Product Size: | 10,000 units |
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| Concentration: | 200 U?μl |
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| Enzyme Function: | RNA dependent DNA Polymerase |
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| Shipping Condition: | Wet Ice |
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| Number of Reactions: | 50 Reactions |
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內容及儲存
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SuperScript? II Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.
Store at -20°C. Guaranteed stable for 6 months when properly stored.
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描述
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SuperScript? II Reverse Transcriptase (RT) is an improved version of SuperScript? RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript? II RT superior performance characteristics.
? Yield – Reduced RNase H activity results in greater first-strand cDNA yields
? Size of cDNA – Generation of RT-PCR products up to 12 kb
? Thermostability – Full activity at 42°C
? Flexibility – Uses either total or poly(A)+ RNA
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.
Unit Definition: One unit of SuperScript? II is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)12-18 as template?primer (3).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 μl for 10 min. at 37°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. ?