貨號：?18080044
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規(guī)格
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| Optimal Reaction Temperature: | 50° C |
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| Final Product Size(s): | 12.3 kb or less |
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| Reverse Transcriptase: | SuperScript? III |
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| Ribonuclease H Activity: | Reduced |
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| Form: | Frozen |
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| Format: | Tube(s) |
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| Quantity: | 10,000 units |
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| Template: | ssRNA |
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| Sensitivity: | Medium |
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| Product Size: | 10,000 units |
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| Concentration: | 200 U?μl |
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| Enzyme Function: | RNA dependent DNA Polymerase |
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| Shipping Condition: | Dry Ice |
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| Number of Reactions: | 50 Reactions |
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內(nèi)容及儲存
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SuperScript? III Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.
Store at -20°C. Guaranteed stable for 6 months when properly stored.
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描述
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SuperScript? III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript? II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA sample. Like SuperScript? II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript? III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.
? Thermostability – Half life of 220 minutes at 50°C
? Yield – Reduced RNase H activity for more full-length cDNA
? Specificity – Full activity at 50°C for increased specificity with Gene Specific Primers
? Applications – Array labeling, cDNA libraries, RT-PCR, primer extension, and 3′ and 5′ RACE
Source: Purified from E. coli expressing the pol gene of M-MLV (1, 2), mutagenized to increase thermal stability and half life and reduce RNase H activity.
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays (Figure 2) and yield and length of cDNA product.
Unit Definition: One unit of SuperScript? III RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)12-18 as a template?primer (3).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 μl for 10 min. at 37°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. ?