?貨號：A29133
規(guī)格
????Promoter:???CMV
??? Cell Line:? CHO-S, SFM Adapted
????Key Function:?? Protein Production, Constitutive Expression
??? Product Size:??? 1 kit
????Delivery Method:???? Transfection
??? Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
描述
????? The ExpiCHO? Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. It is based on high-density culture of ExpiCHO-S? cells in ExpiCHO? Expression Medium. Transient expression is powered by ExpiFectamine? CHO transfection reagent, designed specifically for high-density CHO suspension culture, in combination with a specialized transfection enhancer and culture feed. All components work together synergistically to generate 2- to 10-fold higher protein yields than the HEK293-based Expi293? Expression System Kit and 20- to 150-fold higher yields than the FreeStyle? MAX CHO Expression System. Expression yields of 1 to 3 g per L of transfected culture have been demonstrated for some antibody and non-antibody proteins.
??? The ExpiCHO Expression System offers:
??? ? Milligram to multigram yields of recombinant protein per liter of culture in 5 to 14 days post-transfection, which are frequently? higher than transient 293-based systems
? Cost-effective transient expression in CHO cells, allowing scientists developing protein biotherapeutics to work with CHO-expressed proteins from start to finish in the drug development process
? A robust alternative mammalian transient expression host, allowing for production of proteins that are difficult to express in HEK293 cells
? Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows
? Scalable expression for cultures from <1 ml="" to="">10 L, thus generating the amount of protein needed for your application
???? The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO? SFM, and an antibody-expressing positive control vector. All components of the ExpiCHO Expression System Kit are also available for purchase separately. All components of the system are animal origin–free.