描述

ExpiCHO? Expression Medium is a GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. It is a core component of the ExpiCHO? Expression System Kit (Cat. No. A29133) for scalable transient protein production.

ExpiCHO Expression Medium:
? Supports growth of suspension CHO cultures to densities of over 2 x 10⁷?cells per mL, with >95% cell viability
? Uses a transfection-compatible, serum-free suspension culture medium that enables high transfection efficiencies of >90% with ExpiFectamine? CHO transfection reagent
? Supports transient transfection of suspension CHO cells at a density of 6 x 10⁶?cells per mL
? Facilitates sustained, high-level expression of high-density transiently transfected cultures, achieving yields of up to 3 g per L of recombinant protein
? Supports growth and transfection of CHO cells in culture formats from <1 ml="" in="" multi-well="" plates="" to="">10 L in benchtop bioreactors
? Does not contain phenol red

ExpiCHO Expression Medium is formulated with GlutaMAX?-I reagent. It is ready to use, with no supplementation required. The chemically-defined formulation results in high reproducibility and lot-to-lot reliability. For highest level transient protein expression, the medium is used in combination with a matched culture feed, ExpiCHO? Feed, which is supplied as part of the ExpiFectamine? CHO Transfection Kit (Cat. No. A29129). ExpiCHO Expression Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a humidified, CO₂?cell culture incubator. However, cultures are scalable from volumes of <1 ml="" in="" multi-well="" plates="" to="">10 L in bioreactors.