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ReadyProbes? 即用型成像試劑

發(fā)布時(shí)間： 2015-12-25

ReadyProbes?試劑是即用型成像溶液，讓您無需繁瑣的計(jì)算、稀釋、移液等步驟，即可完成細(xì)胞染色。您只需用滴瓶在每毫升樣品中簡(jiǎn)單加入兩滴，即可進(jìn)入下一實(shí)驗(yàn)步驟。目前已研發(fā)出細(xì)胞核與肌動(dòng)蛋白的染料、細(xì)胞活性與凋亡染料、二抗以及其他成像輔助試劑——部分產(chǎn)品還采用了用戶友好的配方，支持室溫保存，可在實(shí)驗(yàn)臺(tái)上隨取隨用。

核染色

成像實(shí)例

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細(xì)胞核染色。使用NucRed? Live647染料對(duì)在MatTek玻璃底培養(yǎng)皿中生長(zhǎng)過夜的hUVEC細(xì)胞進(jìn)行染色。
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ReadyProbes? 試劑儲(chǔ)存條件檢測(cè)通道 Ex/Em 貨號(hào)

NucBlue? Live Cell
Stain Room Temperature (RT) (≤25°C) DAPI 358/461 R37605

NucBlue? Fixed Cell
Stain RT (≤25°C) DAPI 352/461 R37606

NucGreen? Dead 488
Reagent RT (≤25°C) FITC 504/523 R37109

Propidium Iodide
Reagent RT (≤25°C) RFP 535/617 R37108

NucRed? Live 647 Cell
Stain RT (≤25°C) Cy?5 638/686 R37106

NucRed? Dead 647 Cell
Stain RT (≤25°C) Cy?5 642/661 R37113

ReadyProbes? 試劑	儲(chǔ)存條件	檢測(cè)通道	Ex/Em	貨號(hào)
NucBlue? Live Cell Stain	Room Temperature (RT) (≤25°C)	DAPI	358/461	R37605
NucBlue? Fixed Cell Stain	RT (≤25°C)	DAPI	352/461	R37606
NucGreen? Dead 488 Reagent	RT (≤25°C)	FITC	504/523	R37109
Propidium Iodide Reagent	RT (≤25°C)	RFP	535/617	R37108
NucRed? Live 647 Cell Stain	RT (≤25°C)	Cy?5	638/686	R37106
NucRed? Dead 647 Cell Stain	RT (≤25°C)	Cy?5	642/661	R37113

Actin 染色

成像實(shí)例

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Actin染色。使用Image-iT? Fixation/Permeabilization Kit對(duì)BPAE細(xì)胞進(jìn)行固定、通透和封固操作。使用anti-tubulin antibody標(biāo)記微管蛋白，隨后通過Alexa Fluor? 594 二抗進(jìn)行檢測(cè)。使用ActinGreen? 488?ReadyProbes? 試劑標(biāo)記肌動(dòng)蛋白，并使用NucBlue? Fixed Cell Stain對(duì)細(xì)胞核染色。
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ReadyProbes? 試劑儲(chǔ)存條件檢測(cè)通道 Ex/Em 貨號(hào)

ActinGreen? 488
試劑室溫 (RT) (≤25°C) FITC 495/518 R37110

ActinRed? 555
試劑 RT (≤25°C) RFP 540/565 R37112

ReadyProbes? 試劑	儲(chǔ)存條件	檢測(cè)通道	Ex/Em	貨號(hào)
ActinGreen? 488 試劑	室溫 (RT) (≤25°C)	FITC	495/518	R37110
ActinRed? 555 試劑	RT (≤25°C)	RFP	540/565	R37112

細(xì)胞活性染色

成像實(shí)例

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細(xì)胞活性鑒定。將活的或經(jīng)0.1% Triton? X-100去垢劑處理的Jurkat細(xì)胞混合，并使用ReadyProbes? Cell Viability Imaging Kit (Blue/Red)中的試劑進(jìn)行染色：加入NucBlue? Live stain與碘化丙啶（每毫升細(xì)胞懸液加入兩滴）孵育15分鐘。使用EVOS? FL 全自動(dòng)細(xì)胞成像系統(tǒng)對(duì)細(xì)胞進(jìn)行成像和分析。NucBlue? Live stain能夠?qū)θ考?xì)胞實(shí)施染色，而碘化丙啶僅對(duì)死亡細(xì)胞進(jìn)行著色。
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ReadyProbes? 試劑儲(chǔ)存條件檢測(cè)通道 Ex/Em 貨號(hào)

NucGreen? Dead 488
Reagent Room temperature (RT) (≤25°C) FITC 504/523 R37109

NucRed? Dead 647 Cell
Stain RT (≤25°C) Cy?5
642/661 R37113

ReadyProbes? Cell
Viability Imaging Kit (Blue/Green RT (≤25°C) DAPI/FITC 360/460
504/523 R37609

ReadyProbes? Cell
Viability Imaging Kit (Blue/Red) RT (≤25°C) DAPI/RFP 360/460
535/617 R37610

ReadyProbes? 試劑	儲(chǔ)存條件	檢測(cè)通道	Ex/Em	貨號(hào)
NucGreen? Dead 488 Reagent	Room temperature (RT) (≤25°C)	FITC	504/523	R37109
NucRed? Dead 647 Cell Stain	RT (≤25°C)	Cy?5	642/661	R37113
ReadyProbes? Cell Viability Imaging Kit (Blue/Green	RT (≤25°C)	DAPI/FITC	360/460 504/523	R37609
ReadyProbes? Cell Viability Imaging Kit (Blue/Red)	RT (≤25°C)	DAPI/RFP	360/460 535/617	R37610

細(xì)胞凋亡試劑

成像實(shí)例

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細(xì)胞凋亡鑒定。HeLa細(xì)胞上樣CellEvent? Caspase 3/7 Green?ReadyProbes? 試劑，隨后采用0.5 μM十字孢堿或?qū)φ杖芤禾幚聿⒎跤?小時(shí)。未處理細(xì)胞（A）僅有微弱熒光，而十字孢堿處理后的細(xì)胞（B）上代表凋亡的熒光著色顯著增強(qiáng)（以綠色顯示）。
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ReadyProbes? 試劑儲(chǔ)存條件檢測(cè)通道 Ex/Em 貨號(hào)

CellEvent? Caspase
3/7 Green 室溫 (RT) (≤25°C) FITC 495/518 R37111

ReadyProbes? 試劑	儲(chǔ)存條件	檢測(cè)通道	Ex/Em	貨號(hào)
CellEvent? Caspase 3/7 Green	室溫 (RT) (≤25°C)	FITC	495/518	R37111

二抗

固定與通透后的細(xì)胞染色。使用Image-iT? Fixation?Permeabilization Kit對(duì)CAKI細(xì)胞進(jìn)行固定和通透處理。使用NucBlue? Fixed Cell Stain對(duì)細(xì)胞核進(jìn)行染色（藍(lán)色），使用ActinGreen? 488 Reagent試劑對(duì)肌動(dòng)蛋白進(jìn)行染色（綠色），anti-OxPhos Complex V inhibitor一抗標(biāo)記，并以Alexa Fluor? 594 山羊抗小鼠IgG抗體進(jìn)行檢測(cè)
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ReadyProbes? 試劑儲(chǔ)存條件檢測(cè)通道 Ex/Em 貨號(hào)

Alexa Fluor? 488 Donkey anti-mouse IgG antibody

2–8°C
FITC

495/518
R37114

Alexa Fluor? 594 Donkey anti-mouse IgG antibody

2–8°C

Texas Red?

590/617
R37115

Alexa Fluor? 488 Goat anti-rabbit IgG antibody

2–8°C
FITC 495/518 R37116

Alexa Fluor? 594 Goat anti-rabbit IgG antibody

2–8°C

Texas Red?

590/617
R37117

Alexa Fluor? 488 Donkey anti-rabbit IgG antibody

2–8°C

FITC

495/518
R37118

Alexa Fluor? 594 Donkey anti-rabbit IgG antibody

2–8°C

Texas Red?

590/617

R37119

Alexa Fluor? 488 Goat anti-mouse IgG antibody
2–8°C FITC

495/518
R37120

Alexa Fluor? 594 Goat anti-mouse IgG antibody
2–8°C

Texas Red?

590/617
R37121

ReadyProbes? 試劑	儲(chǔ)存條件	檢測(cè)通道	Ex/Em	貨號(hào)
Alexa Fluor? 488 Donkey anti-mouse IgG antibody	2–8°C	FITC	495/518	R37114
Alexa Fluor? 594 Donkey anti-mouse IgG antibody	2–8°C	Texas Red?	590/617	R37115
Alexa Fluor? 488 Goat anti-rabbit IgG antibody	2–8°C	FITC	495/518	R37116
Alexa Fluor? 594 Goat anti-rabbit IgG antibody	2–8°C	Texas Red?	590/617	R37117
Alexa Fluor? 488 Donkey anti-rabbit IgG antibody	2–8°C	FITC	495/518	R37118
Alexa Fluor? 594 Donkey anti-rabbit IgG antibody	2–8°C	Texas Red?	590/617	R37119
Alexa Fluor? 488 Goat anti-mouse IgG antibody	2–8°C	FITC	495/518	R37120
Alexa Fluor? 594 Goat anti-mouse IgG antibody	2–8°C	Texas Red?	590/617	R37121

成像輔助試劑

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采用Image-iT? 固定/通透試劑盒固定和通透處理的U-2 OS細(xì)胞。表達(dá) CellLight? Mitochondria-GFP的U-2 OS細(xì)胞采用Image-iT? 固定/通透試劑盒固定和通透處理。肌動(dòng)蛋白纖維采用Alexa Fluor? 594 鬼筆環(huán)肽染色，而細(xì)胞核采用NucBlue? 活細(xì)胞染料染色。??
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ReadyProbes? 試劑滴瓶包裝存儲(chǔ)條件活細(xì)胞/固定細(xì)胞貨號(hào)

BackDrop? Background
Suppressor Yes Room Temperature (RT) (≤25°C) Live R37603

Live Cell Imaging
Solution No RT (≤25°C) Live A14291DJ

Image-iT?
Fixation/Permeabilization Kit No RT (≤25°C) Fixed R37602

Image-iT? FX Signal
Enhancer Yes RT (≤25°C) Fixed R37107

LIVE/DEAD? Cell
Imaging Kit (488/570) No –20°C Live R37601

ReadyProbes? 試劑	滴瓶包裝	存儲(chǔ)條件	活細(xì)胞/固定細(xì)胞	貨號(hào)
BackDrop? Background Suppressor	Yes	Room Temperature (RT) (≤25°C)	Live	R37603
Live Cell Imaging Solution	No	RT (≤25°C)	Live	A14291DJ
Image-iT? Fixation/Permeabilization Kit	No	RT (≤25°C)	Fixed	R37602
Image-iT? FX Signal Enhancer	Yes	RT (≤25°C)	Fixed	R37107
LIVE/DEAD? Cell Imaging Kit (488/570)	No	–20°C	Live	R37601