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產(chǎn)品名稱(chēng)：學(xué)習(xí)網(wǎng)購(gòu)操作指南，輕松成為網(wǎng)購(gòu)達(dá)人

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產(chǎn)品特點(diǎn)：產(chǎn)品資料下載包括最新產(chǎn)品信息、最受歡迎的產(chǎn)品信息以及各個(gè)產(chǎn)品的說(shuō)明書(shū) 細(xì)胞生物學(xué)產(chǎn)品和資源指南細(xì)胞重編程工具 Gibco國(guó)產(chǎn)細(xì)胞培養(yǎng)基 Lipofectamine3000 Dynal磁珠多能干細(xì)胞單細(xì)胞傳代方法 mRNA轉(zhuǎn)染試劑貨號(hào)12301D--dynamag_magnets 貨號(hào)12321D-Dynabeads_cell_separation_guide 貨號(hào)12321D-dynamag_magnets 貨號(hào)65001Dynabeads MyOne Streptavidin C1 貨號(hào)65001--IVD Assay Development - OEM Capabilities with Dynabeads 貨號(hào)4306737-Engineering Diagram- MicroAmp? Optical 96-Well Reaction Plates 貨號(hào)4311320-Brochures & Specifications-OpenArray® technology on the QuantStudio 12K Flex Real-Time PCR System(English) 貨號(hào)4311320-Brochures & Specifications-QuantStudio 12K Flex REal-Time PCR System with OpenArray Block(English) 貨號(hào)10342020PCR Enzyme Selection Guide 貨號(hào)10378016Penicillin-Streptomycin-Glutam 貨號(hào)10687010Selection Reagents Brochure 貨號(hào)10744019--Defined Keratinocyte-SFM (1X), liquid 貨號(hào)10777019-RNaseOUT Recombinant Ribonuclease Inhibitor, 5000 units 貨號(hào)10777019-SP6 RNA Polymerase 貨號(hào)10777019-T7 RNA Polymerase 貨號(hào)10828028-Embryonic Stem Cell Culture 貨號(hào)10828028-Feeder-Dependent Cultu...

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GeneArt? CRISPR Nuclease Vector with CD4 Enrichment Kit

貨號(hào)： A21175 規(guī)格 Promoter: CMV, U6 Reporter: CD4 Key Function: Gene Editing, Stable Cell Line Development, Genome Engineering, Episomal Expression Product Size: 10 reactions Cloning Method: Restriction Enzyme ? MCS Delivery Method: Transfection Protein Tag or Fusion: CD4 fusion 內(nèi)容及儲(chǔ)存 Contains:? CRISPR CD4 Nuclease Vector, linearized? 10X Oligonucleotide Annealing Buffer? DNase/RNase-free Water? 5X Ligation Buffer? T4 DNA Ligase? U6 Forward Sequencing Primer? Control Double-stranded OligonucleotideStore in freezer (-5 to -30°C). 描述 The GeneArt® CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads® CD4 magnetic beads. Transfection ...

2015 - 06 - 01

GeneArt? CRISPR Nuclease Vector with CD4 Enrichment Kit

貨號(hào)： A21175 規(guī)格 Promoter: CMV, U6 Reporter: CD4 Key Function: Gene Editing, Stable Cell Line Development, Genome Engineering, Episomal Expression Product Size: 10 reactions Cloning Method: Restriction Enzyme ? MCS Delivery Method: Transfection Protein Tag or Fusion: CD4 fusion 內(nèi)容及儲(chǔ)存 Contains:? CRISPR CD4 Nuclease Vector, linearized? 10X Oligonucleotide Annealing Buffer? DNase/RNase-free Water? 5X Ligation Buffer? T4 DNA Ligase? U6 Forward Sequencing Primer? Control Double-stranded OligonucleotideStore in freezer (-5 to -30°C). 描述 The GeneArt® CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads® CD4 magnetic beads. Transfection ...

2015 - 06 - 01

TRIzol? Reagent

貨號(hào)： 15596018 規(guī)格 Sample Type (General): Bacteria, Yeast, Blood, Plant Samples, Tissue, Cells, Viral Samples High Throughput Compatibility: Not High Throughput-Compatible (Manual) Downstream Application: Reverse Transcriptase PCR (RT-PCR), Cloning, Real-Time Quantitative PCR (qPCR), Nuclease Protection Assays, cDNA Library Construction, Northern Blotting Scale: Scaleable System: TRIzol Quantity: 1 Product Size: 200 mL Final Product: Total RNA, micro RNA, Transcriptome RNA Purification Time: 1 hr Number of Reactions: 200 Preps Isolation Technology: Organic Extraction Starting Material (Amount): Up to 1 x 10^7 cells, Up to 1 g tissue Shipping Condition: Room Temperature 內(nèi)容及儲(chǔ)存包括TRIzol® 試劑。置于2°C至8°C保存。描述 TRIzol® 試劑是一種完全的即用型試劑，可用于提取高質(zhì)量的總RNA或者從各種生物樣本中同時(shí)提取RNA、DNA和蛋白質(zhì)。這種酚和異硫氰酸胍單相溶液，可用于提取人、動(dòng)物、植物、酵母或細(xì)菌來(lái)源的細(xì)胞和組織樣本中的純RNA、DNA和蛋白質(zhì)，只需一個(gè)小時(shí)即可完成。? 從一份樣品同時(shí)提取RNA、DNA和蛋白質(zhì)? 即便是難以處...

2015 - 05 - 27

Lipofectamine? 2000 Transfection Reagent

貨號(hào)： 11668019 規(guī)格 Cell Type: Established Cell Lines, Hard-to-Transfect Cells, Stem Cells, Primary Cells Sample Type (Specific): Plasmid DNA, RNAi Plasmids (shRNA, miR), Synthetic siRNA Transfection Technique: Lipid-Based Transfection Serum Compatible: Yes Product Size: 1.5 mL Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice High Throughput Compatibility: High Throughput-Compatible Green Features: Sustainable packaging 內(nèi)容及儲(chǔ)存置于+4°C保存。Lipofectamine? 2000試劑正確儲(chǔ)存時(shí)可穩(wěn)定保存6個(gè)月。描述 Lipofectamine® 2000轉(zhuǎn)染試劑是一種多用途轉(zhuǎn)染試劑，可在各種貼壁和懸浮細(xì)胞系中提供高效轉(zhuǎn)染。研究人員可使用Lipofectamine® 2000試劑進(jìn)行基于siRNA和shRNA的基因敲除實(shí)驗(yàn)及基因表達(dá)研究。Lipofectamine® 2000是Life Technologies提供的用于siRNA和質(zhì)粒DNA共轉(zhuǎn)染的最佳試劑。采用Lipofectamine® 2000試劑，您可以實(shí)現(xiàn)：? 在各種細(xì)胞系中具有卓越的轉(zhuǎn)染效率和高重組蛋白表達(dá)水平 (表1)? 出眾的siRNA和質(zhì)粒DNA共轉(zhuǎn)染性能? 在血清存在時(shí)亦具有可靠的轉(zhuǎn)染效率 – 轉(zhuǎn)染后無(wú)需更換培養(yǎng)基? 適用于高通量應(yīng)用的可靠性能? 建立穩(wěn)定細(xì)胞系的最佳選擇適用于基因表達(dá)和基因沉默的高性能轉(zhuǎn)染試劑Lipofectamine® 2000試劑適合所有常見(jiàn)細(xì)胞系及許多難以轉(zhuǎn)染的細(xì)胞系，可用于含有或不含血清的培養(yǎng)基。在基因沉默應(yīng)用中...

2015 - 05 - 27

PURELINK RNA MINI KIT

貨號(hào)： 12183020 規(guī)格 Sample Type (General):Bacteria, Yeast, Blood, Tissue, Plant Samples, CellsHigh Throughput Compatibility:Not High Throughput-Compatible (Manual)Downstream Application:Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction, Nuclease Protection Assays, Next-Generation Sequencing, Microarray Analysis, Real-Time Quantitative PCR (qPCR)Scale:MiniSystem:PureLink?Quantity:10 columnsProduct Size:10 prepsFinal Product:Total RNAYield (up to):1000 µgNumber of Reactions:10 PrepsElution Volume - max:300 µlElution Volume - min:30 µlIsolation Technology:Silica Spin ColumnStarting Material (Amount):Up to 200 mg tissue, 5 x 10^6 to 1 x 10^8 cellsShipping Condition:Room Temperature內(nèi)容及儲(chǔ)存 The PureLink® RNA Mini Kit comes with enough RNA lysis solution, spin cartridges, wash tubes, recovery tubes, wash buffers, and RNase-free water to perform 10 RNA purifications. All components sho...

2015 - 05 - 25

About us 公司簡(jiǎn)介

貨號(hào)： C10228 內(nèi)容及儲(chǔ)存 Contents: One box of 50 Countess® Cell Counting Chamber Slides. Each slide contains two cell counting chambers. 75 mm x 25 mm x 1.8 mm; chamber depth = 100 µm. For use at room temperature under normal laboratory conditions. 描述 Countess® 細(xì)胞計(jì)數(shù)板專(zhuān)為Countess® 自動(dòng)細(xì)胞計(jì)數(shù)儀設(shè)計(jì)。 Countess® 計(jì)數(shù)板是一次性的。每張細(xì)胞計(jì)數(shù)板含有兩個(gè)細(xì)胞計(jì)數(shù)室。此包裝包含50片裝細(xì)胞計(jì)數(shù)板1盒和2 x 1 mL 的臺(tái)盼藍(lán)染色液(0.4%)。即用型，方便您使用每張Countess® 細(xì)胞計(jì)數(shù)板都是獨(dú)立包裝并且滅菌的，即時(shí)可用。Countess® 計(jì)數(shù)板是塑料的，一次性的。在細(xì)胞計(jì)數(shù)室中央計(jì)數(shù)。計(jì)數(shù)體積是0.4 µL, 是標(biāo)準(zhǔn)血球計(jì)數(shù)板1-mm2 計(jì)數(shù)面積的4倍。每張計(jì)數(shù)板有兩個(gè)獨(dú)立的小室，可以計(jì)數(shù)同一樣品的重復(fù)，也可以對(duì)兩個(gè)不同的樣品計(jì)數(shù)。使用Countess® 自動(dòng)細(xì)胞計(jì)數(shù)儀只需簡(jiǎn)單的三個(gè)步驟:1. 將10 µL 樣品和10 µL臺(tái)盼藍(lán)混合后加樣到一次性Countess® 細(xì)胞計(jì)數(shù)板.2. 將計(jì)數(shù)板插入到儀器中。3. 按下 "Count cells" 按鍵。就是這么簡(jiǎn)單！在30秒內(nèi)計(jì)數(shù)總細(xì)胞數(shù)，細(xì)胞存活率及細(xì)胞平均大小。獲得準(zhǔn)確的細(xì)胞計(jì)數(shù)。Countess® 計(jì)數(shù)板可與常規(guī)的生化廢物一同處理。與血球計(jì)數(shù)板不同的是，Countess® 計(jì)數(shù)板無(wú)需在使用前后進(jìn)行清潔。

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