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內(nèi)容及儲存
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The 2x mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 10 X 5 mL tube, sufficient for 5000 20-μL reactions (10 μL Master Mix per reaction).
Store at 2–8°C.
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
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描述
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PowerUp? SYBR? Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp? SYBR? Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.
PowerUp? SYBR? Green Master Mix features include:
? A dual hot-start mechanism for excellent specificity
? Highly reproducible CTs over a broad dynamic range
? Inclusion of UDG to help prevent carryover contamination
? Stability of pre-assembled reactions for up to 72 hours
? Compatibility with most real-time qPCR instruments
Exceptional specificity with dual hot-start mechanism
Specificity is paramount in obtaining high-quality data in SYBR? Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp? SYBR? Green Master Mix uses the Dual-Lock? Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp? SYBR? Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification.
Tight reproducibility in CTs over a wide dynamic range
PowerUp? SYBR? Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested (see figure). In addition, this master mix provides efficient amplification over 6 logs of sample input. Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp? SYBR? Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality (see figure).
UDG included for carryover contamination control
The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp? SYBR? Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives.
Stability of pre-assembled reactions
The tight control of the Dual-Lock? Taq DNA polymerase in PowerUp? SYBR? Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data (see figure). This consistency allows users to have confidence and flexibility in the use of PowerUP? SYBR? Green Master Mix across numerous workflow scenarios1.
Broad instrument compatibility
PowerUp? SYBR? Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems? real-time PCR instruments2. It is also compatible with the Bio-Rad IQ?5, Roche LightCycler? LC480, and Stratagene? MX3005P? systems. For optimal results, recommended primer concentrations are 300–800 nM.