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ReadyProbes? 即用型成像試劑

發(fā)布時間： 2015-12-25

ReadyProbes?試劑是即用型成像溶液，讓您無需繁瑣的計算、稀釋、移液等步驟，即可完成細胞染色。您只需用滴瓶在每毫升樣品中簡單加入兩滴，即可進入下一實驗步驟。目前已研發(fā)出細胞核與肌動蛋白的染料、細胞活性與凋亡染料、二抗以及其他成像輔助試劑——部分產(chǎn)品還采用了用戶友好的配方，支持室溫保存，可在實驗臺上隨取隨用。

核染色

成像實例

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細胞核染色。使用NucRed? Live647染料對在MatTek玻璃底培養(yǎng)皿中生長過夜的hUVEC細胞進行染色。
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ReadyProbes? 試劑儲存條件檢測通道 Ex/Em 貨號

NucBlue? Live Cell
Stain Room Temperature (RT) (≤25°C) DAPI 358/461 R37605

NucBlue? Fixed Cell
Stain RT (≤25°C) DAPI 352/461 R37606

NucGreen? Dead 488
Reagent RT (≤25°C) FITC 504/523 R37109

Propidium Iodide
Reagent RT (≤25°C) RFP 535/617 R37108

NucRed? Live 647 Cell
Stain RT (≤25°C) Cy?5 638/686 R37106

NucRed? Dead 647 Cell
Stain RT (≤25°C) Cy?5 642/661 R37113

ReadyProbes? 試劑	儲存條件	檢測通道	Ex/Em	貨號
NucBlue? Live Cell Stain	Room Temperature (RT) (≤25°C)	DAPI	358/461	R37605
NucBlue? Fixed Cell Stain	RT (≤25°C)	DAPI	352/461	R37606
NucGreen? Dead 488 Reagent	RT (≤25°C)	FITC	504/523	R37109
Propidium Iodide Reagent	RT (≤25°C)	RFP	535/617	R37108
NucRed? Live 647 Cell Stain	RT (≤25°C)	Cy?5	638/686	R37106
NucRed? Dead 647 Cell Stain	RT (≤25°C)	Cy?5	642/661	R37113

Actin 染色

成像實例

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Actin染色。使用Image-iT? Fixation/Permeabilization Kit對BPAE細胞進行固定、通透和封固操作。使用anti-tubulin antibody標記微管蛋白，隨后通過Alexa Fluor? 594 二抗進行檢測。使用ActinGreen? 488?ReadyProbes? 試劑標記肌動蛋白，并使用NucBlue? Fixed Cell Stain對細胞核染色。
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ReadyProbes? 試劑儲存條件檢測通道 Ex/Em 貨號

ActinGreen? 488
試劑室溫 (RT) (≤25°C) FITC 495/518 R37110

ActinRed? 555
試劑 RT (≤25°C) RFP 540/565 R37112

ReadyProbes? 試劑	儲存條件	檢測通道	Ex/Em	貨號
ActinGreen? 488 試劑	室溫 (RT) (≤25°C)	FITC	495/518	R37110
ActinRed? 555 試劑	RT (≤25°C)	RFP	540/565	R37112

細胞活性染色

成像實例

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細胞活性鑒定。將活的或經(jīng)0.1% Triton? X-100去垢劑處理的Jurkat細胞混合，并使用ReadyProbes? Cell Viability Imaging Kit (Blue/Red)中的試劑進行染色：加入NucBlue? Live stain與碘化丙啶（每毫升細胞懸液加入兩滴）孵育15分鐘。使用EVOS? FL 全自動細胞成像系統(tǒng)對細胞進行成像和分析。NucBlue? Live stain能夠?qū)θ考毎麑嵤┤旧?，而碘化丙啶僅對死亡細胞進行著色。
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ReadyProbes? 試劑儲存條件檢測通道 Ex/Em 貨號

NucGreen? Dead 488
Reagent Room temperature (RT) (≤25°C) FITC 504/523 R37109

NucRed? Dead 647 Cell
Stain RT (≤25°C) Cy?5
642/661 R37113

ReadyProbes? Cell
Viability Imaging Kit (Blue/Green RT (≤25°C) DAPI/FITC 360/460
504/523 R37609

ReadyProbes? Cell
Viability Imaging Kit (Blue/Red) RT (≤25°C) DAPI/RFP 360/460
535/617 R37610

ReadyProbes? 試劑	儲存條件	檢測通道	Ex/Em	貨號
NucGreen? Dead 488 Reagent	Room temperature (RT) (≤25°C)	FITC	504/523	R37109
NucRed? Dead 647 Cell Stain	RT (≤25°C)	Cy?5	642/661	R37113
ReadyProbes? Cell Viability Imaging Kit (Blue/Green	RT (≤25°C)	DAPI/FITC	360/460 504/523	R37609
ReadyProbes? Cell Viability Imaging Kit (Blue/Red)	RT (≤25°C)	DAPI/RFP	360/460 535/617	R37610

細胞凋亡試劑

成像實例

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細胞凋亡鑒定。HeLa細胞上樣CellEvent? Caspase 3/7 Green?ReadyProbes? 試劑，隨后采用0.5 μM十字孢堿或?qū)φ杖芤禾幚聿⒎跤?小時。未處理細胞（A）僅有微弱熒光，而十字孢堿處理后的細胞（B）上代表凋亡的熒光著色顯著增強（以綠色顯示）。
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ReadyProbes? 試劑儲存條件檢測通道 Ex/Em 貨號

CellEvent? Caspase
3/7 Green 室溫 (RT) (≤25°C) FITC 495/518 R37111

ReadyProbes? 試劑	儲存條件	檢測通道	Ex/Em	貨號
CellEvent? Caspase 3/7 Green	室溫 (RT) (≤25°C)	FITC	495/518	R37111

二抗

固定與通透后的細胞染色。使用Image-iT? Fixation?Permeabilization Kit對CAKI細胞進行固定和通透處理。使用NucBlue? Fixed Cell Stain對細胞核進行染色（藍色），使用ActinGreen? 488 Reagent試劑對肌動蛋白進行染色（綠色），anti-OxPhos Complex V inhibitor一抗標記，并以Alexa Fluor? 594 山羊抗小鼠IgG抗體進行檢測
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ReadyProbes? 試劑儲存條件檢測通道 Ex/Em 貨號

Alexa Fluor? 488 Donkey anti-mouse IgG antibody

2–8°C
FITC

495/518
R37114

Alexa Fluor? 594 Donkey anti-mouse IgG antibody

2–8°C

Texas Red?

590/617
R37115

Alexa Fluor? 488 Goat anti-rabbit IgG antibody

2–8°C
FITC 495/518 R37116

Alexa Fluor? 594 Goat anti-rabbit IgG antibody

2–8°C

Texas Red?

590/617
R37117

Alexa Fluor? 488 Donkey anti-rabbit IgG antibody

2–8°C

FITC

495/518
R37118

Alexa Fluor? 594 Donkey anti-rabbit IgG antibody

2–8°C

Texas Red?

590/617

R37119

Alexa Fluor? 488 Goat anti-mouse IgG antibody
2–8°C FITC

495/518
R37120

Alexa Fluor? 594 Goat anti-mouse IgG antibody
2–8°C

Texas Red?

590/617
R37121

ReadyProbes? 試劑	儲存條件	檢測通道	Ex/Em	貨號
Alexa Fluor? 488 Donkey anti-mouse IgG antibody	2–8°C	FITC	495/518	R37114
Alexa Fluor? 594 Donkey anti-mouse IgG antibody	2–8°C	Texas Red?	590/617	R37115
Alexa Fluor? 488 Goat anti-rabbit IgG antibody	2–8°C	FITC	495/518	R37116
Alexa Fluor? 594 Goat anti-rabbit IgG antibody	2–8°C	Texas Red?	590/617	R37117
Alexa Fluor? 488 Donkey anti-rabbit IgG antibody	2–8°C	FITC	495/518	R37118
Alexa Fluor? 594 Donkey anti-rabbit IgG antibody	2–8°C	Texas Red?	590/617	R37119
Alexa Fluor? 488 Goat anti-mouse IgG antibody	2–8°C	FITC	495/518	R37120
Alexa Fluor? 594 Goat anti-mouse IgG antibody	2–8°C	Texas Red?	590/617	R37121

成像輔助試劑

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采用Image-iT? 固定/通透試劑盒固定和通透處理的U-2 OS細胞。表達 CellLight? Mitochondria-GFP的U-2 OS細胞采用Image-iT? 固定/通透試劑盒固定和通透處理。肌動蛋白纖維采用Alexa Fluor? 594 鬼筆環(huán)肽染色，而細胞核采用NucBlue? 活細胞染料染色。??
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ReadyProbes? 試劑滴瓶包裝存儲條件活細胞/固定細胞貨號

BackDrop? Background
Suppressor Yes Room Temperature (RT) (≤25°C) Live R37603

Live Cell Imaging
Solution No RT (≤25°C) Live A14291DJ

Image-iT?
Fixation/Permeabilization Kit No RT (≤25°C) Fixed R37602

Image-iT? FX Signal
Enhancer Yes RT (≤25°C) Fixed R37107

LIVE/DEAD? Cell
Imaging Kit (488/570) No –20°C Live R37601

ReadyProbes? 試劑	滴瓶包裝	存儲條件	活細胞/固定細胞	貨號
BackDrop? Background Suppressor	Yes	Room Temperature (RT) (≤25°C)	Live	R37603
Live Cell Imaging Solution	No	RT (≤25°C)	Live	A14291DJ
Image-iT? Fixation/Permeabilization Kit	No	RT (≤25°C)	Fixed	R37602
Image-iT? FX Signal Enhancer	Yes	RT (≤25°C)	Fixed	R37107
LIVE/DEAD? Cell Imaging Kit (488/570)	No	–20°C	Live	R37601